INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS

INDUCTION OF ENZYME COCKTAILS BY LOW COST CARBON SOURCES FOR PRODUCTION OF MONOSACCHARIDE-RICH SYRUPS FROM PLANT MATERIALS

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The production of cellulases, hemicellulases, and starch-degrading enzymes by the thermophilic aerobic fungus Talaromyces emersonii under Exfoliant liquid state culture on various food wastes was investigated.A comprehensive enzyme screening was conducted, which resulted in the identification of spent tea leaves as a potential substrate for hydrolytic enzyme production.The potent, polysaccharide-degrading enzyme-rich cocktail produced when tea leaves were utilised as sole carbon source was analysed at a protein and mRNA level and shown to exhibit high level production of key cellulose and hemicellulose Nettle Leaf degrading enzymes.As presented in this paper, the crude enzyme preparation produced after 120 h growth of Talaromyces emersonii on used tea leaves is capable of hydrolysing other lignocellulosic materials into their component monosaccharides, generating high value sugar syrups with a host of industrial applications including conversion to fuels and chemicals.